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Recombinant Human APOE3 Protein

$ 89.00$ 250.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10522
Species: Human
Applications: Bioactive
Available Tags: His
Available Hosts: E. Coli
Purity: Greater than 95% as determined by SDS-PAGE.
Length (Amino Acids):

Size
Tag Clear selection
Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10522-100ug
Recombinant Human APOE3 General Information
APOE3  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Apolipoprotein E, LDLCQ5, APO-E, LPG, AD2, Alzheimer Disease 2 (APOE*E4-Associated, Late Onset), Apolipoprotein E3, APOE.
Molecular Weight
35 kDa
Chromosomal Location
q13.32 on chromosome 19
Curated Database and Bioinformatic Data
Gene SymbolAPOE
Entrez Gene ID348
UniProt ID(s)P02649
COSMIC ID Link(s)APOE
KEGG Gene ID(s)hsa:348
General Description of Recombinant Human APOE3.
Human APOE3 (19-317) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 306 amino acids and having a molecular mass of 35kDa. The APOE is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Human APOE3 Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant APOE3 based upon sequence from Human
Host: QP10522 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of APOE3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized APOE in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Immobilized rHuApoE3 binds to rMuVLDLR with EC50 less than 0.4µg/ml.
Amino Acid Sequence: MHHHHHHKVE QAVETEPEPE LRQQTEWQSG QRWELALGRF WDYLRWVQTL SEQVQEELLS SQVTQELRAL MDETMKELKA YKSELEEQLT PVAEETRARL SKELQAAQAR LGADMEDVCG RLVQYRGEVQ AMLGQSTEEL RVRLASHLRK LRKRLLRDAD DLQKRLAVYQ AGAREGAERG LSAIRERLGP LVEQGRVRAA TVGSLAGQPL QERAQAWGER LRARMEEMGS RTRDRLDEVK EQVAEVRAKL EEQAQQIRLQ AEAFQARLKS WFEPLVEDMQ RQWAGLVEKV QAAVGTSAAP VPSDNH
Purity: Greater than 95% as determined by SDS-PAGE.
Endotoxin Levels:
Buffer: Lyophilized from a 0.2µm filtered solution in PBS.
Storage Conditions: Lyophilized APOE3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOE should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human APOE3 Protein