other Avidin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Avidin based upon sequence from other
Host: QP10525 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Avidin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Avidin in sterile 18Mâ¦.cm H2O not less than 100Âµg/ml or more than 10 mg/ml solutions.
Bioactivity Data: 16 units/mg protein, 1 unit binds 1Âµg biotin.
Amino Acid Sequence: MVHATSPLLL LLLLSLALVA PGLSARKCSL TGKWTNDLGS NMTIGAVNSR GEFTGTYITA VTATSNEIKE SPLHGTQNTI NKRTQPTFGF TVNWKFSEST TVFTGQCFID RNGKEVLKTM WLLRSSVNDI GDDWKATRVG INIFTRLRTQ KE
Purity: Greater than 92.0%.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Storage Conditions: Lyophilized Avidin although stable at room temperature for 3 weeks, should be stored desiccated below -18Â°C. Upon reconstitution Avidin should be stored at 4Â°C between 2-7 days and for future use below -18Â°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered white lyophilized powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.