Bovine BTC Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant BTC based upon sequence from Bovine
Host: QP10540 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BTC was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized BTC Bovine in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is
Amino Acid Sequence: MARAAPGSGA SPLPLLPALA LGLVILHCVV ADGNSTRSPE DDGLLCGDHA ENCPATTTQP KRRGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYA GARCERVDLF YLRGDRGQIL VICLIAVMVI FIILVVSICT CCHPLRKRRK RRKKEEEMET LGKDITPIND DIQETSIA
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Bovine BTC Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard.
Buffer: The Betacellulin Bovine Recombinant was lyophilized after extensive dialysis against 50mM acetic acid.
Storage Conditions: Lyophilized Betacellulin Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BTC Bovine should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.