other DUT Pyrococcus Fruriosus Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant DUT Pyrococcus Fruriosus based upon sequence from other
Host: QP10580 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of DUT Pyrococcus Fruriosus was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Definition of Unit (for specific activity): One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
(A) Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepes pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma. (B) Enhancing PCR amplification: 50ul of Pfu PCR reaction system with 1-3u of dUTPase (<3.0kb) or 4-6u dUTPase (>3.0kb) to amplify genomic DNA target up to 15-19 kb in length. (High concentrations of dUTPase will inhibit PCR reaction!).
Specific Activity: 10Â³u/ug.
Purity: Greater than 97.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: dUTPase is supplied in 20mM Tris-HCl (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at a concentration of 10U/Âµl of the enzyme.
Storage Conditions: Store at 4Â°C if entire vial will be used within 2-4 weeks. Store, frozen at -20Â°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered liquid formulation.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.