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Recombinant Human EGF Protein

$ 89.00$ 250.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10582
Species: Human
Applications: Bioactive
Available Tags: Untagged
Available Hosts: Pichia Pastoris
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Product NumberDescriptionPrice
QP10582-100ug Size: 100 ug, Tag: Untagged (Native Protein) $ 89.00
QP10582-500ug Size: 500 ug, Tag: Untagged (Native Protein) $ 130.00
QP10582-1mg Size: 1 mg, Tag: Untagged (Native Protein) $ 250.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP10582-100ug
Recombinant Human EGF General Information
Alternate Names
Urogastrone, URG, EGF.
Molecular Weight
6 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)Q6QBS2
General Description of Recombinant Human EGF.
Human Epidermal Growth Factor produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa. The EGF is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Human EGF Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant EGF based upon sequence from Human
Host: QP10582 protein expressed in Pichia Pastoris.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of EGF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is
Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Human EGF Protein: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Endotoxin Levels:
Buffer: Each lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH 7.5.
Storage Conditions: Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human EGF Protein