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Recombinant Carp GH Protein

$ 89.00$ 2,100.00
Please Select A Product To View The SKU.

SKU: QP10631
Species: Carp
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10631-10ug
Recombinant Carp GH General Information
Alternate Names
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Molecular Weight
21.4 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)P10298
General Description of Recombinant Carp GH.
Growth Hormone Carp Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 188 amino acids & having a molecular mass of 21,408 Dalton. Growth Hormone Carp is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Carp GH Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant GH based upon sequence from Carp
Host: QP10631 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth Hormone Carp recombinant in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Carp GH is biologically active in rat 3T3 F442A preadipocytes, though its activity is 15-fold lower compared to bovine GH, but it is equally potent in vivo in promoting carp growth (Fine et al. 1993). Furthermore, carp GH forms 1:2 complex with the extra cellular domain of ovine growth hormone receptor.
Amino Acid Sequence: MARVLVLLSV VLVSLLVNQG RASDNQRLFN NAVIRVQHLH QLAAKMINDF EDSLLPEERR QLSKIFPLSF CNSDYIEAPA GKDETQKSSM LKLLRISFHL IESWEFPSQS LSGTVSNSLT VGNPNQLTEK LADLKMGISV LIQACLDGQP NMDDNDSLPL PFEDFYLTMG ENNLRESFRL LACFKKDMHK VETYLRVANC RRSLDSNCTL
Purity: Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels:
Buffer: The GH Carp was lyophilized from a concentrated (1 mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8.
Storage Conditions: Lyophilized Growth Hormone Carp recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Hormone Carp should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Selected References

Growth Hormone Promotes Hair Cell Regeneration in the Zebrafish (Danio rerio) Inner Ear following Acoustic Trauma.Publication:Sun H, Lin C-H, Smith ME (2011) Growth Hormone Promotes Hair Cell Regeneration in the Zebrafish (Danio rerio) Inner Ear following Acoustic Trauma. PLoS ONE 6(11): e28372. doi:10.1371/journal.pone.0028372Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028372

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Carp GH Protein