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Recombinant Gilthead Seabream GH Protein

$ 89.00$ 2,400.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10633
Species: Gilthead Seabream
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10633-10ug
Recombinant Gilthead Seabream GH General Information
Alternate Names
GH1, GH, GHN, GH-N, hGH-N, Pituitary growth hormone, Growth hormone 1, Somatotropin.
Molecular Weight
21.4 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)P29971
General Description of Recombinant Gilthead Seabream GH.
Somatotropin Gilthead Seabream Recombinant Sparus Aurata produced in E. Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21.4 kDa. The Gilthead Seabream Growth-Hormone Recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Gilthead Seabream GH Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant GH based upon sequence from Gilthead Seabream
Host: QP10633 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth-Hormone Gilthead Seabream in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Bioactivity Data: Binding assays of the 125I-labeled gilthead seabream GH to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant gilthead seabream Growth Hormone, like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to Sparus aurata larvae or intraperitoneally to juvenile fish.
Amino Acid Sequence: MDRVVLMLSV MSLGVSSQPI TDGQRLFSIA VSRVQHLHLL AQRLFSDFES SLQTEEQRQL NKIFLQDFCN SDYIISPIDK HETQRSSVLK LLSISYRLVE SWEFPSRSLS GGSAPRNQIS PKLSELKTGI HLLIRANEDG AEIFPDSSAL QLAPYGNYYQ SLGTDESLRR TYELLACFKK DMHKVETYLT VAKCRLSPEA NCTL
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels:
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02% NaHCO3.
Storage Conditions: Lyophilized Growth-Hormone Gilthead Seabream although stable at room temperature for at least two weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH can be stored at 4°C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Gilthead Seabream GH Protein