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Recombinant Bovine GH Protein

$ 89.00$ 675.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10639
Species: Bovine
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10639-20ug
Recombinant Bovine GH General Information
Alternate Names
BGH, BST, rBGH, rBST, Bovine Somatotropin, Bovine GH, Growth hormone, GH1.
Molecular Weight
21.8 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)P01246
General Description of Recombinant Bovine GH.
Growth Hormone Bovine Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.8 kDa. The GH Bovine Recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Bovine GH Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant GH based upon sequence from Bovine
Host: QP10639 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Bovine Growth Hormone in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
Amino Acid Sequence: 1 afpamslsgl fanavlraqh lhqlaadtfk efertyipeg qrysiqntqv 51 afcfsetmpa ptgkneaqqk sdlellrisl lliqswlgpl qflsrvftns 101 lvfgtsdrvy eklkdleegi lalmreledg tprrgqilkq tydkfdtnmr 151 sddallknyg llscfrkdlh ktetylrvmk crrfgeasca f
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Bovine GH Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Endotoxin Levels:
Buffer: The Bovine Growth Hormone protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH 8-9.
Storage Conditions: Lyophilized Bovine Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GH Bovine should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Bovine GH Protein