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Recombinant Human IGF1 Protein

$ 89.00$ 225.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10680
Species: Human
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10680-100ug
Recombinant Human IGF1 General Information
IGF1  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Molecular Weight
7.6 kDa
Chromosomal Location
q23.2 on chromosome 12
Curated Database and Bioinformatic Data
Gene SymbolIGF1
Entrez Gene ID3479
UniProt ID(s)P01343
COSMIC ID Link(s)IGF1
KEGG Gene ID(s)hsa:3479
General Description of Recombinant Human IGF1.
Human Insulin-Like Growth Factor-I produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa. IGF-I is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Human IGF1 Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant IGF1 based upon sequence from Human
Host: QP10680 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IGF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg.
Amino Acid Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.
Endotoxin Levels:
Buffer: The protein was lyophilized from a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0, 130mM NaCl.
Storage Conditions: Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Selected References

1.Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis. Publication: Journal of Endocrinology (2009) 202, 287–297. Link: http://joe.endocrinology-journals.org/content/202/2/287.full.pdfApplication: support spermatogenesis in an androgen-independent manner (Leal et al. 2006)2.Suppression of Anoikis by SKP2 Amplification and Overexpression Promotes Metastasis of Esophageal Squamous Cell Carcinoma .Publication:doi: 10.1158/1541-7786.MCR-08-0092 Mol Cancer Res January 2009 7; 12 Link:http://mcr.aacrjournals.org/content/7/1/12.full3.MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.Publication:THE UNIVERSITY OF TEXAS AT ARLINGTONMay 2010Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502D_10646.pdf?sequence=1 4. Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet.com/JOCM%205(3)%2073-78.pdf

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human IGF1 Protein