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Recombinant Porcine IL 1 alpha Protein

$ 89.00$ 1,300.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10685
Species: Porcine
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10685-2ug
Recombinant Porcine IL 1 alpha General Information
IL 1 alpha  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha, IL1, IL-1A, IL1F1.
Molecular Weight
18.1 kDa
Chromosomal Location
On chromosome 3
Curated Database and Bioinformatic Data
Gene SymbolIL1A
Entrez Gene ID397094
UniProt ID(s)P18430
COSMIC ID Link(s)IL1A
KEGG Gene ID(s)ssc:397094
General Description of Recombinant Porcine IL 1 alpha.
Interleukin-1A Porcine Recombinant produced in E. Coli is single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. The IL-1A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Porcine IL 1 alpha Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant IL 1 alpha based upon sequence from Porcine
Host: QP10685 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 1 alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependant stimulation of D10S cells is
Amino Acid Sequence: MAKVPDLFED LKNCYSENEE YSSDIDHLSL NQKSFYDASY EPLPGDGMDK FMPLSTSKTS KTSRLNFKDS VVMAAANGKI LKKRRLSLNQ FITDDDLEAI ANDTEEEIIK PRSATYSFQS NMKYNFMRVI NHQCILNDAR NQSIIRDPSG QYLMAAVLNN LDEAVKFDMA AYTSNDDSQL PVTLRISETR LFVSAQNEDE PVLLKELPET PKTIKDETSL LFFWEKHGNM DYFKSAAHPK LFIATRQEKL VHMAPGLPSV TDFQILENQS
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Porcine IL 1 alpha Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.
Endotoxin Levels:
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.
Storage Conditions: Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Porcine IL 1 alpha Protein