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Recombinant Rat IL 1 alpha Protein

$ 89.00$ 4,680.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10686
Species: Rat
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10686-2ug
Recombinant Rat IL 1 alpha General Information
IL 1 alpha  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Molecular Weight
17.7 kDa
Chromosomal Location
q36 on chromosome 3
Curated Database and Bioinformatic Data
Gene SymbolIl1a
Entrez Gene ID24493
UniProt ID(s)P16598
COSMIC ID Link(s)Il1a
KEGG Gene ID(s)rno:24493
General Description of Recombinant Rat IL 1 alpha.
Rat Interleukin-1A produced in E. Coli is single, a non-glycosylated, Polypeptide chain containing 155 amino acids and having a molecular mass of 17703 Dalton. The IL-1A is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Rat IL 1 alpha Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant IL 1 alpha based upon sequence from Rat
Host: QP10686 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 1 alpha was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin 1a in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the dose-dependant stimulation of murine D10S cells is
Amino Acid Sequence: MAKVPDLFED LKNCYSENEE YSSAIDHLSL NQKSFYDASY GSLHENCTDK FVSLRTSETS KMSTFTFKES RVVVSATSNK GKILKKRRLS FNQPFTEDDL EAIAHDLEET IQPRSAPHSF QNNLRYKLIR IVKQEFIMND SLNQNIYVDM DRIHLKAASL NDLQLEVKFD MYAYSSGGDD SKYPVTLKVS NTQLFVSAQG EDKPVLLKEI PETPKLITGS ETDLIFFWEK INSKNYFTSA AFPELLIATK EQSQVHLARG LPSMIDFQIS
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels:
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing 50mM Tris-HCl, pH=8.
Storage Conditions: Lyophilized Interleukin-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Rat IL 1 alpha Protein