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Recombinant Mouse Leptin Protein

$ 89.00$ 2,850.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10756
Species: Mouse
Applications: Bioactive
Available Tags: 20kD PEG, Untagged
Available Hosts: E. Coli
Purity: Greater than 99.0% as determined by:(a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10756-PEG-5ug
Recombinant Mouse Leptin General Information
Alternate Names
OB Protein, Obesity Protein, OBS, Obesity factor.
Curated Database and Bioinformatic Data
UniProt ID(s)P41160
General Description of Recombinant Mouse Leptin.
Mouse Leptin Mono-PEGylated produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and an additional Ala at N-terminus. PEGylated Mouse Leptin contains PEG 20 kDa at its N-terminus and having a molecular mass of 35.6 kDa as determined by mass spectrometry. Since its enlarged hydrodynamic volume PEGylated Leptin runs on SDS-PAGE as A 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. PEGylated Mouse Leptin half-life in circulation after SC injection was over 20 hours. Mouse Leptin was purified by using an optimized multi-step FPLC method for maximum separation from contaminants. according to Salomon et al (2006) Protein Expression and Purification 47.128–136 and then PEGylated.

Mouse Leptin Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant Leptin based upon sequence from Mouse
Host: QP10756 protein expressed in E. Coli.
Available Tags: 20kD PEG, Untagged
Protein Construction: A cDNA sequence encoding the sequence of Leptin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Leptin in sterile 0.4% NaHCO3 adjusted to pH 8.5 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Pegylated mouse Leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Pegylated mouse Leptin in vitro activity is only slightly lower than the non-pegylated antagonist but in vivo it has profound weight reducing effect (as compared to the non-pegylated leptin), resulting mainly from reduced food intake.
Amino Acid Sequence: MCWRPLCRFL WLWSYLSYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
Purity: Greater than 99.0% as determined by:(a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
Endotoxin Levels:
Buffer: The mouse Leptin was lyophilized from a concentrated (0.65 mg/ml) solution containing 0.003mM NaHCO3.
Storage Conditions: Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Mouse Leptin Protein