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Recombinant Ovine Leptin qA Protein

$ 98.00$ 1,888.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP10759
Species: Ovine
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 98.0% as determined by:(a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Length (Amino Acids):

Product NumberDescriptionPrice
QP10759-10ug : 10-ug: untagged $ 98.00
QP10759-50ug : 50-ug: untagged $ 148.00
QP10759-1mg : 1-mg: untagged $ 1,888.00
Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP10759-10ug

Ovine Leptin qA Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant Leptin qA based upon sequence from Ovine
Host: QP10759 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Leptin qA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: enQuire Bio’s leptin qA is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Purity: Greater than 98.0% as determined by:(a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Concentration of Ovine Leptin qA Protein: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Endotoxin Levels:
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 LEP-qA mutant mg/ml and up to 2 mg/ml and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Physical Appearance: White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Ovine Leptin qA Protein