other LLO Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant LLO based upon sequence from other
Host: QP10773 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of LLO was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Other Applications: (1) Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA 4. (2) Production of specific monoclonal antibodies5. (3) Detection of anti-listeriolysin O antibodies6.
Bioactivity Data: Hemolytic activity for Recombinant Listeriolysin O was 1.18 x 105 HU/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay. 2mM DTT is used to reactivate the toxin.
Amino Acid Sequence: MKKIMLVFIT LILISLPIAQ QTEAKDASAF HKEDLISSMA PPTSPPASPK TPIEKKHADE IDKYIQGLDY NKNNVLVYHG DAVTNVPPRK GYKDGNEYIV VEKKKKSINQ NNADIQVVNA ISSLTYPGAL VKANSELVEN QPDVLPVKRD SLTLSIDLPG MTNQDNKIVV KNATKSNVNN AVNTLVERWN EKYAQAYPNV SAKIDYDDEM AYSESQLIAK FGTAFKAVNN SLNVNFGAIS EGKMQEEVIS FKQIYYNVNV NEPTRPSRFF GKAVTKEQLQ ALGVNAENPP AYISSVAYGR QVYLKLSTNS HSTKVKAAFD AAVSGKSVSG DVELTNIIKN SSFKAVIYGG SAKDEVQIID GNLGDLRDIL KKGATFNRET PGVPIAYTTN FLKDNELAVI KNNSEYIETT SKAYTDGKIN IDHSGGYVAQ FNISWDEINY DPEGNEIVQH KNWSENNKSK LAHFTSSIYL PGNARNINVY AKECTGLAWE WWRTVIDDRN LPLVKNRNIS IWGTTLYPKY SNSVDNPIE
Purity: Greater than 90.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% (v/v) glycerol and 0.5M NaCl.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.