Lysostaphin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Lysostaphin based upon sequence from other
Host: QP10778 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Lysostaphin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted to other aqueous solutions such as 200mM Tris HCL pH 8 for optimal activity.
Bioactivity Data: Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH 8, 30°C. Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor.
Specific Activity: Determined to be 3,542 units/mg.
Amino Acid Sequence: MKKTKNNYYT RPLAIGLSTF ALASIVYGGI QNETHASEKS NMDVSKKVAE VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKAPV ENTAEVETSK APVENTAEVE TSKAPVENTA EVETSKAPVE NTAEVETSKA PVENTAEVET SKAPVENTAE VETSKAPVEN TAEVETSKAP VENTAEVETS KAPVENTAEV ETSKAPVENT AEVETSKALV QNRTALRAAT HEHSAQWLNN YKKGYGYGPY PLGINGGMHY GVDFFMNIGT PVKAISSGKI VEAGWSNYGG GNQIGLIEND GVHRQWYMHL SKYNVKVGDY VKAGQIIGWS GSTGYSTAPH LHFQRMVNSF SNSTAQDPMP FLKSAGYGKA GGTVTPTPNT GWKTNKYGTL YKSESASFTP NTDIITRTTG PFRSMPQSGV LKAGQTIHYD EVMKQDGHVW VGYTGNSGQR IYLPVRTWNK STNTLGVLWG TIK
Purity: 97% as determined by RP-HPLC.
Concentration of other Lysostaphin Protein: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized without any additives.
Storage Conditions: Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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