Rat PDGF AA Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PDGF AA based upon sequence from Rat
Host: QP10803 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PDGF AA was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in Sterile 4mM HCl to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Bioactivity Data: The ED50, as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 Ã 105 IU/mg.
Monomer or Dimer: Dimer
Amino Acid Sequence: MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R
Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Buffer: PDGF-AA was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.0.
Storage Conditions: Lyophilized Platelet-derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.