Human Transferrin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Transferrin based upon sequence from Human
Host: QP10893 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Transferrin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes Recombinant Human Transferrin has increased the growth of numerous cell types including hybridoma and primary cell lines. The optimal concentration of Recombinant Human Transferrin for cell culture differs with the cell line. In CHO, effective concentrations range from 10-50 mg/liter. The reported effective concentrations for hybridoma and Vero cell lines range from 5 mg/liter to 20 mg/liter. Effective concentrations for primary cells range between 5 mg/liter to 100 mg/liter.
Recommended Reconstitution Instructions: Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5 mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2µm filter.
Iron Content: The average amount of Ferric Iron in our Transferrin is 0.4-0.5ug per mg of protein (protein concentration is measured by absorption at 280 nm).
Why We Recommend this protein: Our Human Transferrin is an animal component-free, well defined and consistent universal iron carrier in cell culture. Replaces plasma-derived human and bovine transferrin and other iron chelators.
Bioactivity Data: One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe.
Amino Acid Sequence: MRLAVGALLV CAVLGLCLAV PDKTVRWCAV SEHEATKCQS FRDHMKSVIP SDGPSVACVK KASYLDCIRA IAANEADAVT LDAGLVYDAY LAPNNLKPVV AEFYGSKEDP QTFYYAVAVV KKDSGFQMNQ LRGKKSCHTG LGRSAGWNIP IGLLYCDLPE PRKPLEKAVA NFFSGSCAPC ADGTDFPQLC QLCPGCGCST LNQYFGYSGA FKCLKDGAGD VAFVKHSTIF ENLANKADRD QYELLCLDNT RKPVDEYKDC HLAQVPSHTV VARSMGGKED LIWELLNQAQ EHFGKDKSKE FQLFSSPHGK DLLFKDSAHG FLKVPPRMDA KMYLGYEYVT AIRNLREGTC PEAPTDECKP VKWCALSHHE RLKCDEWSVN SVGKIECVSA ETTEDCIAKI MNGEADAMSL DGGFVYIAGK CGLVPVLAEN YNKSDNCEDT PEAGYFAIAV VKKSASDLTW DNLKGKKSCH TAVGRTAGWN IPMGLLYNKI NHCRFDEFFS EGCAPGSKKD SSLCKLCMGS GLNLCEPNNK EGYYGYTGAF RCLVEKGDVA FVKHQTVPQN TGGKNPDPWA KNLNEKDYEL LCLDGTRKPV EEYANCHLAR APNHAVVTRK DKEACVHKIL RQQQHLFGSN VTDCSGNFCL FRSETKDLLF RDDTVCLAKL HDRNTYEKYL GEEYVKAVGN LRKCSTSSLL EACTFRRP
Purity: Purity as determined by SDS-PAGE is 95%.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein (1 mg/ml) was lyophilized with no additives.
Storage Conditions: Lyophilized Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Transferrin Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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