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Recombinant Rat IL 13, Partial Protein

$ 89.00$ 4,680.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP14019
Species: Rat
Applications: Bioactive
Available Tags: Untagged
Available Hosts: E. Coli
Purity: Greater than 95% as determined by SDS-PAGE.
Length (Amino Acids):

Size
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Shipping Information
1-3 days
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QP14019-EC-2ug
Recombinant Rat IL 13, Partial General Information
IL 13  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
NC300, ALRH, BHR1, P600, IL-13.
Molecular Weight
12.7 kDa
Chromosomal Location
q22 on chromosome 10
Curated Database and Bioinformatic Data
Gene SymbolIl13
Entrez Gene ID116553
UniProt ID(s)P42203
COSMIC ID Link(s)Il13
KEGG Gene ID(s)rno:116553
General Description of Recombinant Rat IL 13, Partial.
Rat Interleukin-13 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 113 amino acids and having a molecular mass of 12.7 kDa. The IL-13 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Rat IL 13, Partial Bioactive Protein Product Attributes

Product Type: Bioactive Protein
Recombinant IL 13, Partial based upon sequence from Rat
Host: QP14019 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of IL 13, Partial was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: ED50 range = 2-6 ng/mL as determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay.
Amino Acid Sequence: MALWVTAVLA LACLGGLATP GPVRRSTSPP VALRELIEEL SNITQDQKTS LCNSSMVWSV DLTAGGFCAA LESLTNISSC NAIHRTQRIL NGLCNQKASD VASSPPDTKI EVAQFISKLL NYSKQLFRYG H
Purity: Greater than 95% as determined by SDS-PAGE.
Concentration of Rat IL 13, Partial Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.
Endotoxin Levels:
Buffer: The protein (1 mg/ml) was lyophilized with no additives.
Storage Conditions: Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Rat IL 13, Partial Protein