Dengue Virus Dengue Rapid Test Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Dengue Rapid Test based upon sequence from Dengue Virus
Host: QP11653 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Dengue Rapid Test was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Other Applications: 1. Prepare 528 to 533nm colloid gold, conjugation within 24 hours after colloid gold preparation;2. Adjust colloid gold OD at 1.3 to 1.4, conjugation concentration and pH are 20ug / ml gold and 7.2;3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20oC for 20 min;4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2;5. Add the supplied blocking reagent to the final concentration to 0.4%;6. Blocking for 1 hours , then add sucrose to the final concentration to 20%;7. Dipping the conjugated gold to the gold pad ( do not use sodium phosphate for gold pad treatment);8. After drying, assemble gold pad and absorbent pad over backing card; 9. Add 10µl serum/plasma and add the supplied running buffer for assay. 10. It is suggested to use our monoclonal anti-human IgG and IgM cat# ant-529 and ant-530 for rapid test development kit.
Bioactivity Data: Untested
Purity: Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Phosphate buffered saline pH 7.4 and 0.02% sodium nitrate.
Storage Conditions: Recombinant Dengue Rapid Test although stable at 4C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
Limitations and Performance Guarantee
enQuire Bio's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.