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Recombinant Human PKC-a Protein

$ 89.00$ 1,100.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP13064
Species: Human
Applications: See Application Notes
Available Tags: His
Available Hosts: Insect
Purity: Greater than 95% as determined by SDS-PAGE.
Length (Amino Acids):

Product NumberDescriptionPrice
QP13064-2ug Size: 2 ug, Tag: His $ 89.00
QP13064-10ug Size: 10 ug, Tag: His $ 130.00
QP13064-0.1mg Size: 0.1 mg, Tag: His $ 1,100.00
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In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP13064-2ug
Recombinant Human PKC-a General Information
PKC-a  protein 3D structural model from Catalog of Somatic Mutations in Cancer originally published in the paper COSMIC: somatic cancer genetics at high-resolution
Alternate Names
Protein kinase C alpha type, EC 2.7. 11.13, PKC-alpha, PKC-A, PRKCA, AAG6, PKCA, PRKACA, MGC129900, MGC129901.
Molecular Weight
76.7 kDa
Chromosomal Location
q24.2 on chromosome 17
Curated Database and Bioinformatic Data
Gene SymbolPRKCA
Entrez Gene ID5578
UniProt ID(s)P17252
COSMIC ID Link(s)PRKCA
KEGG Gene ID(s)hsa:5578
General Description of Recombinant Human PKC-a.
Human Protein Kinase C alpha produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 76.7 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag. PKC-a is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

Human PKC-a Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant PKC-a based upon sequence from Human
Host: QP13064 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of PKC-a was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): 7200- 9000 units/mg. One unit of PKC-a activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30°C. Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3b. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions.
Additional Information: Dilute in 10mM HEPES (pH7.4), 0.01% CHAPS and 5mM DTT. *for further dilution you may use either the Storage Buffer or Dilution Buffer.
Bioactivity Data:
Specific Activity: 1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 2ug/ml.
Amino Acid Sequence: MADVYPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKHPAKR LGCGPEGERD VREHAFFRGI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: PKC-a is supplied in 20mM HEPES pH 7.5, 250mM NaCl, 50% glycerol, 2mM EDTA, 2mM EGTA, 0.05%Triton X-100 and 5mM DTT.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Colorless Liquid

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

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Recombinant Human PKC-a Protein