Rat PVALB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant PVALB based upon sequence from Rat
Host: QP13219 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PVALB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:A- Dilute 1 µl of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MSMTDLLSAE DIKKAIGAFT AADSFDHKKF FQMVGLKKKS ADDVKKVFHI LDKDKSGFIE EDELGSILKG FSSDARDLSA KETKTLMAAG DKDGDGKIGV EEFSTLVAES
Purity: Greater than 90% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated solution (1 mg/ml) without any additives.
Storage Conditions: Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Parvalbumin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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