Placeholder

Recombinant TEV Protein

$ 89.00$ 250.00
Please Select Product Options Below To View The Catalog Number.

SKU: QP13725
Species: other
Applications: See Application Notes
Available Tags: His
Available Hosts: E. Coli
Purity: Greater than 90.0% as determined by analysis by SDS-PAGE.
Length (Amino Acids):

Product NumberDescriptionPrice
QP13725-100IU Size: 100IU, Tag: His $ 89.00
QP13725-500IU Size: 500IU, Tag: His $ 130.00
QP13725-1,000IU Size: 1000IU, Tag: His $ 250.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QP13725-100IU
Recombinant other TEV General Information
Alternate Names
rTEV, TEV, P1 protease.
Molecular Weight
34.8 kDa
Curated Database and Bioinformatic Data
UniProt ID(s)Q0GDU8
COSMIC ID Link(s)-
General Description of Recombinant other TEV.
Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30°C; however, the enzyme can be used at temperatures as low as 4°C. The rTEV contains His tag. The rTEV is purified by using an optimized multi-step FPLC method for maximum separation from contaminants.

other TEV Recombinant Protein Product Attributes

Product Type: Recombinant Protein
Recombinant TEV based upon sequence from other
Host: QP13725 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of TEV was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.
Application Notes: A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV.
Bioactivity Data: Untested
Amino Acid Sequence: GHIVWPDYAN ILKEVFGGAR MACVTSAHMA GANGSILKKA EETSRATMHK PVIFGEDYVT EADLPYTPLH LEVNAEMERM YYLGRRALTH GKRRKVSVNN KRNRRRKVAK TYVGRDSIVE KIVVPHTERK VDTTTAVKDT CNEVSTQLVH NSMPKRKKQK NFLPATSLSN VYAQTWSIVR KRHMQVEIIS KKSVRAKVKR FEGSVQLFAS VRHMYGERKR VDLRIDNWQQ KTLLDLAKRF KNERVDQSKL TFGSSGLVLR QGSYAPAHWY RHGMFIVRGR SDGMLVDARA KVTFAVCYSM THY
Purity: Greater than 90.0% as determined by analysis by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The rTEV contains 25mM Tris, 75mM NaCl, 5mM EDTA, 10mM GSH, 50% Glycerol.
Storage Conditions: rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Physical Appearance: Sterile liquid formulation.

Limitations and Performance Guarantee

enQuire Bio's products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.

There are no reviews yet.

Be the first to review “Recombinant TEV Protein”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Recombinant TEV Protein