Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RB Ab (PD7/26).

Anti-CD45RB Antibody Clone PD7/26

Name: Anti-CD45RB Antibody Clone PD7/26
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 488.00

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SKU: 5788-MSM4
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
5788-MSM4-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 5788-MSM4-P1

CD45RB General Information
Alternate Names
Protein tyrosine phosphatase, receptor type, C, PTPRC
Molecular Weight
180-220kDa
Chromosomal Location
1q31.3
Curated Database and Bioinformatic Data
Gene Symbol	PTPRC
Entrez Gene ID	5788
Ensemble Gene ID	ENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)	XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)	XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)	NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)	X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)	X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)	9666
Cosmic ID(s)	PTPRC
KEGG Gene ID(s)	hsa:5788
PharmGKB ID(s)	PA34011
General Description of CD45RB.
CD45R, also designated CD45, PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5,, 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6), CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes, the majority of lymphomas, leukemias of B-cell origin.

Human Anti-CD45RB Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-CD45RB Antibody Clone: PD7/26

Clone PD7/26 Host and Isotype: Mouse IgG1 kappa

Anti-Human CD45RB Positive Control Sample: Ramos, U-698, or GA-10 cells. Tonsil

Cellular Localization of Antibody Cell surface, cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

CD45RB Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RB antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RB could be seen in. We were unable to detect CD45RB in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RB expression as measured by anti-CD45RB antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	-	-	-	-	-	+++	-	-	-	-	-	-	-	-	-	-
PTPRC Variability	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Limitations and Warranty

enQuire Bio's CD45RB Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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