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Flow Cytometry Protocol

Prepare your cell suspensions for Flow Cytometry Although most flow cytometry experiments involve labeling populations of cells that are relatively abundant, the number of cells required will vary depending upon the rarity of your cells. In general, researchers will stain between 1 x 105 and 1 x 106 cells per sample. However, with exceeding rare...

Femto Chemiluminescent Substrate [ELISA] – Discontinued

SKU: QAB99 Species: Mouse Tested Applications: Flow Cytometry, immunoprecipitation Available Conjugates: Unconjugated Isotype: Golden Syrian Hamster IgG Mass Spec Validated?: Not MS Validated

Femto Chemiluminescent Substrate [WB]

Analysis of Protein Array containing >19,000 full-length human proteins using CD68 Mouse Monoclonal Antibody (C68/2501) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

CD68 (Macrophage Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 968-MSM7
Species: Human
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2c, kappa
Mass Spec Validated?: Not MS Validated

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD68 Mouse Monoclonal Antibody (LAMP4/1830) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

CD68 (Macrophage Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 968-MSM6
Species: Human
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b, kappa
Mass Spec Validated?: Not MS Validated

SDS-PAGE Analysis Purified CD8 Mouse Recombinant Monoclonal Antibody (rC8/468).Confirmation of Purity and Integrity of Antibody.

CD8A (Cytotoxic-&Suppressor T-Cell Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 925-MSM11
Species: Human
Tested Applications: Flow, IF, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Flow Cytometry of human Vimentin on Jurkat Cells.Black: Cells alone; Grey: Isotype Control.Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).

Anti-Human Vimentin, Monospecific Antibody Clone VM452

SKU: 7431-MSM1 Species: Human, Cow, Dog, Cat, Pig, Goat and Chicken. Does not react with mouse and rat. Tested Applications: Flow, IF, WB, IHC Available Conjugates: Unconjugated Isotype: Mouse IgG1 Mass Spec Validated?: Not MS Validated

SDS-PAGE Analysis Purified p53 Recombinant Rabbit Monoclonal Antibody (TP53/2092R).Confirmation of Purity and Integrity.

p53 Tumor Suppressor Protein Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 7157-RBM17
Species: Human, Monkey, Dog, Hamster and Chicken. Does not react with mouse and rat.
Tested Applications: Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Rabbit IgG
Mass Spec Validated?: Not MS Validated

SDS-PAGE Analysis Purified Thrombomodulin Mouse Recombinant MAb (rTHBD/1591).Confirmation of Purity and Integrity of Antibody.

Thrombomodulin / CD141 (Endothelial Cell Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 7056-MSM3
Species: Human
Tested Applications: ELISA, Flow, IF, WB, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Analysis of Protein Array containing more than 19,000 full-length human proteins using BCL-6 Mouse Monoclonal Antibody (BCL6/1982).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Bcl-6 (Follicular Lymphoma Marker) Ultraspecific Antibody
Tested against >20,000 Human Proteins

SKU: 604-MSM8
Species: Human
Tested Applications: Flow, IF, IHC
Available Conjugates: Unconjugated
Isotype: Mouse IgG1, kappa
Mass Spec Validated?: Not MS Validated

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