Validation Project Details

MS QAVA™:  Quantitative Measurement of Antibody Specificity Powered by Mass Spec.

Existing validation only provides approximations and assumptions of specificity. Here’s how it works:

Analysis of Mass Spec data (dashed-line) of fractions stained with EGFR (Her1 / ErbB1) QAVA mAb [Clone H9B4] (solid line) reveals that less than 10% of signal is attributable to non-specific binding of H9B4 to targets other than EGFR (Her1 / ErbB1). This specificity is excellent compared to the average antibody which shows as much as 30% non-specific signal. Data from Jurkat, U2oS and HeLa cells.
Analysis of Mass Spec data (dashed-line) of fractions stained with EGFR (Her1 / ErbB1) QAVA mAb [Clone H9B4] (solid line) reveals that less than 10% of signal is attributable to non-specific binding of H9B4 to targets other than EGFR (Her1 / ErbB1). This specificity is excellent compared to the average antibody which shows as much as 30% non-specific signal. Data from Jurkat, U2oS and HeLa cells.
Mass Spec Antibody Validation
Analysis of Mass Spec data (dashed-line) of fractions stained with p21-WAF1 QAVA mAb [Clone HJ21] (solid line) reveals less than 10% of signal is attributable to non-specific binding of p21-WAF1. This specificity is excellent compared to the industry average, which is near 30% non-specific binding.

Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared.  r-squared represents variability in antibody signal attributable to variability in level of protein (as measured by mass spec). Unexplained variability described as non-specific antibody signal. Further details available. Collaborator data in press. Please contact us for additional info.

 

The average antibody has over 30% non-specific signal. Get more accurate staining with our first MS-QAVA validated antibodies. Click here..

Testing Every Antibody We Sell.


enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

Validation Project Details