MS QAVA™: Quantitative Measurement of Antibody Specificity Powered by Mass Spec.
Existing validation only provides approximations and assumptions of specificity. Here’s how it works:
Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared. r-squared represents variability in antibody signal attributable to variability in level of protein (as measured by mass spec). Unexplained variability described as non-specific antibody signal. Further details available. Collaborator data in press. Please contact us for additional info.
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