MS QAVA™: Quantitative Measurement of Antibody Specificity Powered by Mass Spec.
Existing validation only provides approximations and assumptions of specificity. Here’s how it works:
Analysis of Mass Spec data (dashed-line) of fractions stained with EGFR (Her1 / ErbB1) QAVA mAb [Clone H9B4] (solid line) reveals that less than 10% of signal is attributable to non-specific binding of H9B4 to targets other than EGFR (Her1 / ErbB1). This specificity is excellent compared to the average antibody which shows as much as 30% non-specific signal. Data from Jurkat, U2oS and HeLa cells.Analysis of Mass Spec data (dashed-line) of fractions stained with p21-WAF1 QAVA mAb [Clone HJ21] (solid line) reveals less than 10% of signal is attributable to non-specific binding of p21-WAF1. This specificity is excellent compared to the industry average, which is near 30% non-specific binding.
Immunoprecipitation and Mass Spec is run for every fraction for a total of 36-72 sample types per antibody. Specificity is calculated as antibody vs. mass spec r-squared. r-squared represents variability in antibody signal attributable to variability in level of protein (as measured by mass spec). Unexplained variability described as non-specific antibody signal. Further details available. Collaborator data in press. Please contact us for additional info.
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![Analysis of Mass Spec data (dashed-line) of fractions stained with EGFR (Her1 / ErbB1) QAVA mAb [Clone H9B4] (solid line) reveals that less than 10% of signal is attributable to non-specific binding of H9B4 to targets other than EGFR (Her1 / ErbB1). This specificity is excellent compared to the average antibody which shows as much as 30% non-specific signal. Data from Jurkat, U2oS and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/07/1956-MSM3-P1-EGFR-Antibody-data-10.png)
