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200 ul of predilute SingleStep™ Poly-HRP conjugated goat anti-mouse secondary antibody was incubated 30 minutes following staining of human liver samples with a hepatocyte specific mitochondrial marker (antibody catalog number msm4-966) Excellent signal intensity is observed in the absence of any additional amplification step.

DISCONTINUED: Anti-Mouse IgG (H+L) Secondary Antibody [SingleStep™ PolyHRP]

SKU: Q2AB1 Species: Mouse Tested Applications: Immunohistochemistry Available Conjugates: SingleStep™ Poly-HRP Isotype: Goat IgG Mass Spec Validated?: Not MS Validated

100 ul of predilute SingleStep™ Poly-HRP conjugated goat anti-rabbit secondary antibody was incubated 30 minutes following anti-EpCAM staining of human rectum samples. Excellent signal intensity is observed in the absence of any additional amplification step.

DISCONTINUED: SingleStep Poly-HRP Anti-Rabbit IgG (H+L) Secondary Antibody

SKU: Q2AB2 Species: Rabbit Tested Applications: Immunohistochemistry Available Conjugates: SingleStep™ Poly-HRP Isotype: Goat IgG Mass Spec Validated?: Not MS Validated

Flow Cytometry Protocol

Prepare your cell suspensions for Flow Cytometry Although most flow cytometry experiments involve labeling populations of cells that are relatively abundant, the number of cells required will vary depending upon the rarity of your cells. In general, researchers will stain between 1 x 105 and 1 x 106 cells per sample. However, with exceeding rare...

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Western blot data demonstrating successful knockdown of BAX by QX9 at 48 hrs post transfection (SC = Scrambled Control (Product Number QC1), siRNA = QX9 treatment)

TranslationBlocker Mouse Apoptosis regulator BAX siRNA – DISCONTINUED

Mouse Apoptosis regulator BAX siRNA Translation Blocker™ Duplex

Flow Cytometry data demonstrating successful knockdown of PD-L2 / CD273 by QX8 at 48 hrs post 2nd transfection (Orange = QX8 siRNA, Blue-Violet = Negative Control siRNA (Product Number QC1)

TranslationBlocker Human PD-L2 / CD273 siRNA – DISCONTINUED

Human PD-L2 / CD273 siRNA Translation Blocker™ Duplex

Flow Cytometry data demonstrating successful knockdown of PD-L1 / CD274 by QX7 at 48 hrs post 2nd transfection (Orange = QX7 siRNA, Blue-Violet = Negative Control siRNA (Product Number QC1), Light Purple = Isotype Control)

TranslationBlocker Human PD-L1 / CD274 siRNA

Human PD-L1 / CD274 siRNA Translation Blocker™ Duplex

Western blot data demonstrating successful knockdown of BAX by QX6 at 48 hrs post transfection (SC = Scrambled Control (Product Number QC1), siRNA = QX6 treatment)

TranslationBlocker Human Apoptosis regulator BAX siRNA

Human Apoptosis Inhibitor BAX siRNA Translation Blocker™ Duplex

Western blot data demonstrating successful knockdown of Insulin Receptor in human cells approximately 72 hours after treatment with QX51 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX51 treatment)

TranslationBlocker Human Insulin Receptor siRNA – DISCONTINUED

Human Insulin Receptor siRNA Translation Blocker™ Duplex

Western blot data demonstrating successful knockdown of mTor in human cells approximately 72 hours after treatment with QX50 siRNA (SC = Scrambled Control (Product Number QC1), siRNA = QX50 treatment)

TranslationBlocker Human mTOR siRNA – DISCONTINUED

Human mTOR siRNA Translation Blocker™ Duplex

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