PDF DatasheetHuman, Mouse, and Rat Anti-ACTH Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunoprecipitation (0.5-1 ug of antibody/500ug protein Lysate), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-ACTH Antibody Clone: AH26 + 57
Clone AH26 + 57 Host and Isotype: Mouse IgG
Anti-Human, Mouse, and Rat ACTH Positive Control Sample: Normal pituitary gland or pituitary tumor.
Cellular Localization of Antibody Cytoplasm
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH (AH26); N-terminal fragment of human ACTH conjugated to KLH (57)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
ACTH Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in ACTH antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the anterior in pituitary gland. More moderate antibody staining intensity was present in cells in the anterior in pituitary gland. Low, but measureable presence of ACTH could be seen in. We were unable to detect ACTH in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| ACTH General Information | |
|---|---|
| Alternate Names | |
| Pro-opiomelanocortin, POMC | |
| Molecular Weight | |
| ACTH is ~5kDa,, the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation, post-translational modifications. | |
| Chromosomal Location | |
| 2p23.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | POMC |
| Entrez Gene ID | 5443 |
| Ensemble Gene ID | ENSG00000115138 |
| RefSeq Protein Accession(s) | NP_001306134, NP_000930, NP_001030333, NP_001306133 |
| RefSeq mRNA Accession(s) | NM_001319204 NM_001035256, NM_001319205, NM_000939 |
| RefSeq Genomic Accession(s) | NG_008997, NC_000002, NC_018913 |
| UniProt ID(s) | P01189 |
| UniGene ID(s) | P01189 |
| HGNC ID(s) | 9201 |
| Cosmic ID(s) | POMC |
| KEGG Gene ID(s) | hsa:5443 |
| PharmGKB ID(s) | PA33526 |
| General Description of ACTH. | |
| ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin, Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells,, macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors, the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. | |
Limitations and Warranty
enQuire Bio’s ACTH Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
![Staining with Mouse Monclonal ACTH [Clone 57] Antibody in formalin-fixed paraffin-embedded human pituitary gland.](https://enquirebio.com/wp-content/uploads/2019/01/ACTH IHC human Pituitary Gland (57)-150x150.jpg)
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![Staining with mouse monoclonal ACTH [Clone 2F6] antibody in formalin-fixed paraffin-embedded human pituitary gland.](https://enquirebio.com/wp-content/uploads/2019/01/ACTH IHC human Pituitary Gland (2F6)-150x150.jpg)
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