Anti-Bcl-2 Antibody Clone BCL2/782 + BCL2/796

$ 429.00

SKU: 596-MSM7
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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Human Anti-Bcl-2 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Bcl-2 Antibody Clone: BCL2/782 + BCL2/796
Clone BCL2/782 + BCL2/796 Host and Isotype: Mouse IgG
Anti-Human Bcl-2 Positive Control Sample: Jurkat, K562, HL-60, or HeLa Cells. Tonsil or follicular lymphomas.
Cellular Localization of Antibody Outer mitochondrial membranes, endoplasmic reticulum as well as nuclear membranes.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human bcl-2 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Bcl-2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-BCL2 antibody staining is expected to be primarily localized to the nuclear membrane and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Bcl-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the white pulp in spleen, glandular cells in the endometrium, epididymis, fallopian tube, prostate, seminal vesicle and thyroid gland, hematopoietic cells in the bone marrow, lymphoid tissue in appendix, melanocytes in skin, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Bcl-2 could be seen inadipocytes in breast and mesenchymal tissue, exocrine glandular cells in the pancreas, glandular cells in the duodenum, gallbladder and small intestine and squamous epithelial cells in the esophagus. We were unable to detect Bcl-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Bcl-2 expression as measured by anti-Bcl-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + +++ +++ ++
BCL2 Variability ++ ++ + ++ ++ ++ + + ++ ++ ++ ++ ++ + ++ ++ + + ++ +

Limitations and Warranty

enQuire Bio’s Bcl-2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-Bcl-2 Antibody Clone BCL2/782 + BCL2/796

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