PDF DatasheetHuman Anti-c-Myc Oncoprotein Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, immunofluorescence, immunoprecipitation, Western Blot.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-c-Myc Oncoprotein Antibody Clone: 9E10
Clone 9E10 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
c-Myc Oncoprotein Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MYC antibody staining is expected to be primarily localized to the nucleoplasm.
| c-Myc Oncoprotein General Information | |
|---|---|
| Alternate Names | |
| c-Myc Oncoprotein, MRTL, MYCC, bHLHe39, c-Myc | |
| Curated Database and Bioinformatic Data | |
| Entrez Gene ID | 4609 |
| Ensemble Gene ID | ENSG00000136997 |
| RefSeq Protein Accession(s) | NP_001341799, NP_002458 |
| RefSeq mRNA Accession(s) | NM_001354870, NM_002467 |
| RefSeq Genomic Accession(s) | NC_000008, NG_007161, NC_018919 |
| UniProt ID(s) | P01106, A0A087WVR4 |
| UniGene ID(s) | P01106, A0A087WVR4 |
| HGNC ID(s) | 7553 |
| Cosmic ID(s) | MYC |
| KEGG Gene ID(s) | hsa:4609 |
| PharmGKB ID(s) | PA31353 |
| General Description of c-Myc Oncoprotein. | |
| The 9E10 antibody was generated using a peptide (EQKLISEEDL) within the C-terminal region of human c-myc, a protein expressed in the cell nucleus, where it plays an important role in cell cycle regulation and apoptosis. In addition, c-myc is considered to be a proto-oncogenic protein as it is expressed at higher levels in a variety of hematopoietic tumors. The 9E10 antibody is widely utilized to purify or identify recombinant proteins which have been engineered to express the 9E10 epitope tag. c-myc tagged proteins may be analyzed by flow cytometry, Western Blot, and ELISA, among many other applications. The epitope detected by 9E10 is highly conserved, allowing this antibody to be used in a variety of species. | |
Selected References
Stengel et al. 2012. Proc. Natl. Acad. Sci. 109: 5399-5404. (Cell sorting / Flow cytometry)Servant et al. 2012. Nucleic Acids Res. 40: 5271-5282. (Chromatin immunoprecipitation /ChIP)Manca et al. 2012. Proc. Natl. Acad. Sci. 109: 2009-2012. (in vitro injection – chicken embryos)Hou et al. 2012. J. Biol. Chem. 287: 474-483. (Western Blot, Immunoprecipitation)Neerincx et al. 2012. J. Immunol. 188: 4940-4950. (Immunofluorescence microscopy)Dodson et al. 2012. Hum. Mol. Genet. 21: 1350-1363. (Immunocytochemistry)Laurent et al. 2012. Nucleic Acids Res. 40: 3159-3171. (RNA-FISH combined with immunofluorescence)Banfield et al. 1994. J. Cell Biol. 127: 357-371. (Electron microscopy)Siegel et al. 1998. Int. J. Oncol. 13(6): 1259-1262. (Western blot – mouse)
Limitations and Warranty
enQuire Bio’s Human Anti-c-Myc Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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