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Antibody (Suitable for clinical applications)
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Calponin Information for Pathologists
Summary:
Actin filament associated regulatory protein. h1 (basic) isoform is smooth muscle specific, but a late stage smooth muscle marker; described below. h2 isoform is found in smooth muscle and non muscle cells; not described below. Uses by pathologists Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202).
Common Uses By Pathologists:
Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202). Help differentiate breast collagenous spherulosis (positive) from adenoid cystic carcinoma (negative, Mod Pathol 2006;19:1351), although adenoid cystic carcinoma is calponin positive in salivary gland tumors (Arch Pathol Lab Med 1999;123:801). Microscopic (histologic) images Images hosted on Pathout server:.
| Calponin General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 33.2 kDa | |
| Chromosomal Location | |
| p13.2 [chr: 19] [chr_start: 11538767] [chr_end: 11550323] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CNN1 |
| Entrez Gene ID | 1264 |
| RefSeq Protein Accession(s) | NP_001295270; NP_001290 |
| RefSeq mRNA Accession(s) | XM_005259741; XM_024451361; NM_001299; NM_001308342; XM_024451362; NM_001308341; XM_017026289 |
| RefSeq Genomic Accession(s) | NC_000019 |
| UniProt ID(s) | P51911 |
| PharmGKB ID(s) | PA26665 |
| KEGG Gene ID(s) | hsa:1264 |
| General Description of Calponin . | |
| Multiple isoelectric variants of calponin have been identified but only two molecular weight isoforms exist (34kDa and 29kDa). Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. | |
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