Calponin [CALP (same as hCP)] Antibody (cGMP) – DISCONTINUED

SKU: QIVD84
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

PDF Datasheet

DISCONTINUED

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.

Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.

Application Notes

Specification	Recommendation
Recommended Dilution (Conc)	1:50-1:100
Pretreatment	Citrate Buffer pH 6.0
Incubation Parameters	30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal

Anti-Calponin Antibody Clone: CALP (same as hCP)

Host and Isotype: Mouse IgG1, kappa

Recommended Positive Control Sample: Uterus

Cellular Localization of Antibody CALP (same as hCP) Staining: Cytoplasmic

Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN₃

Antibody Concentration: Lot specific. Plese contact tech support for data.

Immunogen: Crude human uterus extract.

Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Calponin Information for Pathologists

Summary:

Actin filament associated regulatory protein. h1 (basic) isoform is smooth muscle specific, but a late stage smooth muscle marker; described below. h2 isoform is found in smooth muscle and non muscle cells; not described below. Uses by pathologists Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202).

Common Uses By Pathologists:

Identify myoepithelial cells in breast lesions (Histopathology 2005;47:202). Help differentiate breast collagenous spherulosis (positive) from adenoid cystic carcinoma (negative, Mod Pathol 2006;19:1351), although adenoid cystic carcinoma is calponin positive in salivary gland tumors (Arch Pathol Lab Med 1999;123:801). Microscopic (histologic) images Images hosted on Pathout server:.

Calponin General Information
Alternate Names

Molecular Weight
33.2 kDa
Chromosomal Location
p13.2 [chr: 19] [chr_start: 11538767] [chr_end: 11550323] [strand: 1]
Curated Database and Bioinformatic Data
Gene Symbol	CNN1
Entrez Gene ID	1264
RefSeq Protein Accession(s)	NP_001295270; NP_001290
RefSeq mRNA Accession(s)	XM_005259741; XM_024451361; NM_001299; NM_001308342; XM_024451362; NM_001308341; XM_017026289
RefSeq Genomic Accession(s)	NC_000019
UniProt ID(s)	P51911
PharmGKB ID(s)	PA26665
KEGG Gene ID(s)	hsa:1264
General Description of Calponin .
Multiple isoelectric variants of calponin have been identified but only two molecular weight isoforms exist (34kDa and 29kDa). Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

Size	0.1 ml, 25 ml, 6 ml, 0.5 ml, 1 ml
Tag	Unconjugated
Buffer and Stabilizer	PBS with 1% BSA and 0.05% NaN3
Product Type	Antibody
Host	Mouse
Isotype	IgG1, kappa
Applications	IHC
Species	Human
Format	Concentrate, Predilute

Size

0.1 ml, 25 ml, 6 ml, 0.5 ml, 1 ml

Tag

Unconjugated

Buffer and Stabilizer

PBS with 1% BSA and 0.05% NaN3

Product Type

Host

Isotype

Applications

Species

Format

Concentrate, Predilute

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