.jpg "Calprotectin IHC human Tonsil (MAC387)")
PDF DatasheetHuman, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse Anti-Calprotectin Antibody Product Attributes
Species: Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Calprotectin Antibody Clone: MAC387
Clone MAC387 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Canine, Rabbit, Guinea pig, Rat, and Mouse Calprotectin Positive Control Sample: Tonsil, lymph node, or spleen
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Affinity purified monocyte membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Calprotectin Previously Observed Antibody Staining Patterns
| Calprotectin General Information | |
|---|---|
| Alternate Names | |
| S100A8, S100A9 Heterodimer, Calprotectin | |
| Molecular Weight | |
| 12-14kDa (doublet) | |
| Chromosomal Location | |
| 1q21 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | S100A8 & S100A9 |
| Entrez Gene ID | , 6280 |
| Ensemble Gene ID | , ENSG00000163220 |
| RefSeq Protein Accession(s) | , NP_002956 |
| RefSeq mRNA Accession(s) | , NM_002965 |
| RefSeq Genomic Accession(s) | , NC_018912, NC_000001 |
| UniProt ID(s) | , P06702 |
| UniGene ID(s) | , P06702 |
| HGNC ID(s) | , 10499 |
| Cosmic ID(s) | , S100A9 |
| KEGG Gene ID(s) | , hsa:6280 |
| PharmGKB ID(s) | , PA34911 |
| General Description of Calprotectin. | |
| Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain, a 14kDa beta chain expressed by granulocytes, monocytes, by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal, inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffers cells), lung (alveolar macrophages), lymph nodes (free, fixed macrophages), spleen (free, fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural, peritoneal macrophages), skin (histiocytes, Langerhans’s cell), in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing, destroying many potential pathogens including bacteria, pathogenic protozoa, fungi, helminthes. This MAb reacts with neutrophils, monocytes, macrophages,, squamous mucosal epithelia, has been shown as an important marker for identifying macrophages in tissue sections. | |
Limitations and Warranty
enQuire Bio’s Calprotectin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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