PDF DatasheetHuman Anti-Carcinoembryonic Antigen / CD66 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Carcinoembryonic Antigen / CD66 Antibody Clone: COL-1
Clone COL-1 Host and Isotype: Mouse IgG2a kappa
Anti-Human Carcinoembryonic Antigen / CD66 Positive Control Sample: MCF7 or 293T cells. Colon carcinoma
Cellular Localization of Antibody Cytoplasmic, luminal surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human colon carcinoma extract
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Carcinoembryonic Antigen / CD66 Previously Observed Antibody Staining Patterns
| Carcinoembryonic Antigen / CD66 General Information | |
|---|---|
| Alternate Names | |
| Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, anti-Carcinoembryonic Antigen-related Cell Adhesion Molecule 5 antibody, anti-CEACAM5 antibody, anti-CD66 antibody | |
| Molecular Weight | |
| 80-200kDa | |
| Chromosomal Location | |
| 19q13.1-19q13.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CEACAM1 & CEACAM5 |
| Entrez Gene ID | 1048, 634 |
| Ensemble Gene ID | ENSG00000105388, ENSG00000079385 |
| RefSeq Protein Accession(s) | XP_016881634, XP_016881635, NP_001278413, NP_004354, XP_005258470, NP_001295327, XP_011524624, NP_001020083, NP_001192273, NP_001171744, NP_001703, NP_001171742, NP_001171745, XP_011525508 |
| RefSeq mRNA Accession(s) | XM_017026145, XM_017026146, XM_011526322, NM_001291484, XM_005258413, NM_001308398, NM_004363, NM_001184815, NM_001184816, NM_001205344, NM_001024912, XM_011527206 NM_001184813, NM_001712 |
| RefSeq Genomic Accession(s) | NC_000019, NC_018930NC_018930, NC_000019, NG_029051 |
| UniProt ID(s) | P06731, Q53G30, A0A024R0K5, 13688, Q3KRG8 |
| UniGene ID(s) | P06731, Q53G30, A0A024R0K5, 13688, Q3KRG8 |
| HGNC ID(s) | 1817, 1814 |
| Cosmic ID(s) | CEACAM5, CEACAM1 |
| KEGG Gene ID(s) | hsa:1048, hsa:634 |
| PharmGKB ID(s) | PA26361, PA26358 |
| General Description of Carcinoembryonic Antigen / CD66. | |
| This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut, is re-expressed in increased amounts in intestinal carcinomas, several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA), with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues, is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma, in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary,, endocervix. | |
Limitations and Warranty
enQuire Bio’s Carcinoembryonic Antigen / CD66 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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