PDF DatasheetMouse Anti-CD117 / c-Kit Antibody Product Attributes
CD117 / c-Kit Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Staining with anti-CD117 / c-Kit antibody reveals CD117 / c-Kit expression is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in CD117 / c-Kit antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD117 / c-Kit could be seen inepidermal cells in the skin. We were unable to detect CD117 / c-Kit in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD117 / c-Kit expression as measured by anti-CD117 / c-Kit antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| KIT Variability | + | ++ | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | ++ | ++ | + |
| CD117 / c-Kit General Information | |
|---|---|
| Alternate Names | |
| CD117, c-Kit, Gsfsco1, Tr-kit, SOW3, Ssm, Bs, Gsfsow3, SCO1, SCO5, CD117, c-KIT, Fdc, Gsfsco5, W | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Kit |
| Entrez Gene ID | 16590 |
| Ensemble Gene ID | ENSMUSG00000005672 |
| RefSeq Protein Accession(s) | NP_001116205, XP_017176176, NP_066922 |
| RefSeq mRNA Accession(s) | XM_017320687, NM_021099, NM_001122733 |
| RefSeq Genomic Accession(s) | NC_000071 |
| UniProt ID(s) | P05532 |
| UniGene ID(s) | P05532 |
| Cosmic ID(s) | Kit |
| KEGG Gene ID(s) | mmu:16590 |
| General Description of CD117 / c-Kit. | |
| The ACK2 antibody is specific for CD117, also called c-Kit, a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.The ACK2 antibody is widely utilized as a marker to identify hematopoietic progenitors, and to neutralize receptor-ligand binding in vitro and in vivo (use format appropriate for functional assays). In addition, the antibody is reported to be cross-reactive with rat c-Kit and is extensively published for use in this species. | |
Selected References
Limitations and Warranty
| Size | |
|---|---|
| Tag | |
| Buffer and Stabilizer | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2, 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
| Product Type | |
| Host | |
| Isotype | |
| Applications | |
| Species | |
| Mass Spec Validated? |
Only logged in customers who have purchased this product may leave a review.
There are no reviews yet.