Anti-CD117 / c-Kit Antibody Clone C117/370 + KIT/982 + KIT/983

$ 488.00

SKU: 3815-MSM4
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

 PDF Datasheet

Human Anti-CD117 / c-Kit Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD117 / c-Kit Antibody Clone: C117/370 + KIT/982 + KIT/983
Clone C117/370 + KIT/982 + KIT/983 Host and Isotype: Mouse IgG
Anti-Human CD117 / c-Kit Positive Control Sample: Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis, mast cells in the dermis of normal skin.
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CD117 proteins
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD117 / c-Kit Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-KIT antibody staining is expected to be primarily localized to the plasma membrane. There is variability in either the signal strength or the localization of signal in plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in CD117 / c-Kit antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD117 / c-Kit could be seen inepidermal cells in the skin. We were unable to detect CD117 / c-Kit in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD117 / c-Kit expression as measured by anti-CD117 / c-Kit antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity
KIT Variability + ++ + + + + + + + + + + + + + + + ++ ++ +

Limitations and Warranty

enQuire Bio’s CD117 / c-Kit Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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Anti-CD117 / c-Kit Antibody Clone C117/370 + KIT/982 + KIT/983

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