Anti-Human/Mouse CD11b Antibody [M1/70]

$ 58.00 – $ 268.00

SKU: QAB22
Species: Human and Mouse
Tested Applications: Flow Cytometry
Available Conjugates: APC ;Unconjugated ;APC-Cy7 ;Biotin ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

PDF Datasheet

Human and Mouse Anti-CD11b Antibody Product Attributes

Species: Human and Mouse

Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque

Tested Applications: Flow Cytometry.

Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization

Clonality: Monoclonal Antibody

Anti-CD11b Antibody Clone: M1/70

Clone M1/70 Host and Isotype: Rat IgG2b kappa

Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2

Antibody Concentration: 0.2 mg/mL

Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11b Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11b antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD11b could be seen in cells in the molecular layer in cerebellum, glial cells in the caudate nucleus, cerebral cortex and hippocampus and macrophages in lung. We were unable to detect CD11b in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fassler R, and Ley K. 2012. Blood. 119:4275-4282. (in vitro blocking)Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy – frozen tissue)Kim W-K, Sun Y, Do H, Autissier P, Halpern EF, Piatak M, Lifson JD, Burdo TH, McGrath MS, and Williams K. 2010. J. Leukoc. Biol. 87: 557-567. (Flow Cytometry – Rhesus macaque)Roland CL, Dineen SP, Lynn KD, Sullivan LA, Dellinger MT, Sadegh L, Sullivan JP, Shames DS, and Brekken RA. 2009. Mol. Cancer Ther. 8:1761-1771. (Immunofluorescence microscopy – frozen tissue)Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry – formalin-fixed paraffin embedded tissue)Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, and Song W-C. 2008. Blood. 112:1109-1119. (in vivo blocking)Ou R, Zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry – OCT embedded frozen tissue)Nutt SL, Metcalf D, DAmico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry – frozen tissue, paraffin embedded tissue)Miller LJ, Schwarting R, and Springer TA. 1986. J. Immunol. 137:2891-2900. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio’s Anti-Human/Mouse CD11b Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

Size	25 ug, 100 ug, 100 tests, 500 ug
Tag	APC, APC-Cy7, Biotin, FITC, PE, PE-Cy5, PE-Cy7, PerCP-Cy5.5, Qfluor™ 630, Qfluor™ 710, Unconjugated, V450
Buffer and Stabilizer	10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2, 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Product Type	Antibody
Host	Rat
Isotype	IgG2b, kappa
Applications	FC
Species	Human and Mouse
Mass Spec Validated?	Not MS Validated

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