PDF DatasheetHuman Anti-Anti-CD152 Antibody Product Attributes
Species: Human
Cross Reactivity: baboon, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-Anti-CD152 Antibody Clone: BNI3
Clone BNI3 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CD152 Previously Observed Antibody Staining Patterns
| Anti-CD152 General Information | |
|---|---|
| Alternate Names | |
| CTLA4, CD152, CTLA-4, ALPS5, GRD4, CD, CD152, IDDM12, GSE, CELIAC3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CTLA4 |
| Entrez Gene ID | 1493 |
| Ensemble Gene ID | ENSG00000163599 |
| RefSeq Protein Accession(s) | NP_005205, NP_001032720 |
| RefSeq mRNA Accession(s) | NM_001037631, NM_005214, |
| RefSeq Genomic Accession(s) | NG_011502, NC_000002, NC_018913 |
| UniProt ID(s) | P16410 |
| UniGene ID(s) | P16410 |
| HGNC ID(s) | 2505 |
| Cosmic ID(s) | CTLA4 |
| KEGG Gene ID(s) | hsa:1493 |
| PharmGKB ID(s) | PA27006 |
| General Description of Anti-CD152. | |
| The BNI3 antibody is specific for human CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions. The BNI3 antibody may be used for flow cytometric analysis of intracellular or surface CTLA-4 expression, and is also widely used for neutralization of CTLA-4 when expressed at the cell surface. The BNI3 antibody is reported to be cross-reactive with Baboon, Cynomolgus and Rhesus CTLA-4. Please choose the appropriate format for each application. | |
Selected References
Moreno-Fernandez ME, Rueda CM, Rusie LK, and Chougnet CA. 2011. Blood. 117: 5372-5380. (in vitro blocking)Schonfeld D, Matschiner G, Chatwell L, Trentmann S, Gille H, Hulsmeyer M, Brown N, Kaye PM, Schlehuber S, Hohlbaum AM and Skerra A. 2009. 106: 8198-8203. (Immunohistochemistry – frozen tissue)Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)Bonzheim I, Geissinger E, Tinguely M, Roth S, Grieb T, Reimer P, Wilhelm M, Rosenwald A, Muller-Hermelink HK, and Rudiger T. 2008. Am. J. Clin. Pathol. 130: 613-619. (Immunohistochemistry – paraffin embedded tissue; Immunofluorescence microscopy – frozen tissue)Young NT, Waller ECP, Patel R, Roghanian A, Austyn JM, and Trowsdale J. 2008. 111: 3090-3096. (in vitro activation)Wei B, da Rocha Dias S, Wang H and Rudd CE. 2007. J. Immunol. 179: 400-408. (in vitro activation)Jonuleit H, Schmitt E, Stassen M, Tuettenberg A, Knop J and Enk AH. 2001. J. Exp. Med. 193: 1285-1294 (in vitro blocking)Oaks MK and Hallett KM. 2000. J. Immunol. 164: 5015-5018. (Immunoprecipitation; EIA – plate coating)
Limitations and Warranty
enQuire Bio’s Human Anti-CD152 / CTLA-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
| Size | |
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| Tag | |
| Buffer and Stabilizer | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2, 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
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| Mass Spec Validated? |
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