PDF DatasheetHuman Anti-CD25 Antibody Product Attributes
Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD25 Antibody Clone: BC96
Clone BC96 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD25 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD25 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD25 could be seen inhematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD25 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD25 General Information | |
|---|---|
| Alternate Names | |
| Interleukin-2 receptor alpha, IL2RA, IL-2RA | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IL2RA |
| Entrez Gene ID | 3559 |
| Ensemble Gene ID | ENSG00000134460 |
| RefSeq Protein Accession(s) | NP_000408, NP_001295172, NP_001295171 |
| RefSeq mRNA Accession(s) | NM_000417, NM_001308242 NM_001308243 |
| RefSeq Genomic Accession(s) | NC_000010, NG_007403, NC_018921 |
| UniProt ID(s) | Q5W005, P01589 |
| UniGene ID(s) | Q5W005, P01589 |
| HGNC ID(s) | 6008 |
| Cosmic ID(s) | IL2RA |
| KEGG Gene ID(s) | hsa:3559 |
| PharmGKB ID(s) | PA29828 |
| General Description of CD25. | |
| The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on induced peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. | |
Selected References
Willer DO, Ambagala APN, Pilon R, chan JK, Fournier J, Brooks J, Sandstrom P, and MacDonald KS. 2012. J. Virol. 86: 3626-3634. (Flow cytometry – cynomolgus)Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)Mackroth MS, Malhotra I, Mungai P, Koech D, Muchiri E, and King CL. 2011. J. Immunol. 186: 2780-2791. (Flow cytometry)Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y, and Yoshimura A. 2011. Int. Immunol. 23(11):701-712. (Cell sorting / negative selection – T cells)Wan Q, Kozhaya L, ElHed A, Ramesh R, Carlson TJ, Djretic IM, Sundrud MS, and Unutmax D. 2011. J. Exp. Med. 208: 1875-1887. (Cell sorting / negative selection – T cells)Verhoeven D, Sankaran S, Silvey M, and Dandekar S. 2008. J. Virol. 82: 4016-4027. (Flow cytometry – rhesus macaque)
Limitations and Warranty
enQuire Bio’s Human Anti-CD25 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
| Size | |
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| Tag | |
| Buffer and Stabilizer | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2, 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
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| Mass Spec Validated? |
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