![Analysis of Mass Spec data (dashed-line) of fractions stained with CD3e MS-QAVA™ monoclonal antibody [Clone: C3e/1308] (solid-line), reveals that less than 1.4% of signal is attributable to non-specific binding of anti-CD3e [Clone: C3e/1308] to targets other than CD3E protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-916-MSM8-P0-anti-CD3e-antibody.png "CD3e Antibody Mass Spec Validation Data")
PDF DatasheetHuman Anti-CD3e Antibody Product Attributes
Species: Human
Tested Applications: Western Blot. Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-CD3e Antibody Clone: C3e/1308
Clone C3e/1308 Host and Isotype: Mouse IgG2b kappa
Anti-Human CD3e Positive Control Sample: Jurkat cells. Tonsil and lymph node
Cellular Localization of Antibody Cell surface and cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CD3e fragment (aa 23-119)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD3e Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Variations in CD3e antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD3e could be seen in. We were unable to detect CD3e in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD3e General Information | |
|---|---|
| Alternate Names | |
| CD3e molecule, Cluster of Differentiation 3E, Often just referred to as CD3 | |
| Molecular Weight | |
| 20kDa | |
| Chromosomal Location | |
| 11q23.3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD3E |
| Entrez Gene ID | 916 |
| Ensemble Gene ID | ENSG00000198851 |
| RefSeq Protein Accession(s) | NP_000724 |
| RefSeq mRNA Accession(s) | NM_000733, |
| RefSeq Genomic Accession(s) | NC_018922, NG_007383, NC_000011 |
| UniProt ID(s) | P07766 |
| UniGene ID(s) | P07766 |
| HGNC ID(s) | 1674 |
| Cosmic ID(s) | CD3E |
| KEGG Gene ID(s) | hsa:916 |
| PharmGKB ID(s) | PA26216 |
| General Description of CD3e. | |
| Recognizes the ?-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. | |
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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