PDF DatasheetHuman, Rabbit, Cow, and Rat Anti-CD5 Antibody Product Attributes
Species: Human, Rabbit, Cow, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD5 Antibody Clone: C5/473 + CD5/54/F6
Clone C5/473 + CD5/54/F6 Host and Isotype: Mouse IgG
Anti-Human, Rabbit, Cow, and Rat CD5 Positive Control Sample: 293T, Ramos, or MOLT-4 Cells. Tonsil.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human CD5 recombinant protein (C5/473); A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD5 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| CD5 General Information | |
|---|---|
| Alternate Names | |
| CD5, Cluster of Differentiation 5 | |
| Molecular Weight | |
| 67kDa | |
| Chromosomal Location | |
| 11q12.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CD5 |
| Entrez Gene ID | 921 |
| Ensemble Gene ID | ENSG00000110448 |
| RefSeq Protein Accession(s) | NP_001333385, NP_055022 |
| RefSeq mRNA Accession(s) | NM_014207, NM_001346456 |
| RefSeq Genomic Accession(s) | NC_018922, NC_000011, |
| UniProt ID(s) | P06127 |
| UniGene ID(s) | P06127 |
| HGNC ID(s) | 1685 |
| Cosmic ID(s) | CD5 |
| KEGG Gene ID(s) | hsa:921 |
| PharmGKB ID(s) | PA26224 |
| General Description of CD5. | |
| Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes, 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma,, a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes. | |
Limitations and Warranty
enQuire Bio’s CD5 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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