Human PBMCs were stained with 5 uL (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL .
Human PBMCs were stained with 5 uL (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL .

Anti-CD5 Antibody [UCHT2]

$ 88.00 – $ 308.00

SKU: QAB15
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;APC ;PerCP-Cy5.5
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

SKU: QAB15-PE-25Tests Categories: , , , , , , Tag:
 PDF Datasheet

Human Anti-CD5 Antibody Product Attributes

Species: Human
Cross Reactivity: capuchin monkey, chimpanzee, common marmoset

Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1×105 to 1×108 cells.
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: UCHT2
Clone UCHT2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD5 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6): 1630-1634. Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223): 535-537. Wood GS and Freudenthal PS. 1992. Am J Pathol. 141(4): 789-795. (Flow Cytometry)

Limitations and Warranty

enQuire Bio’s Human Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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