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Antibody (Suitable for clinical applications)
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:10-1:25 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
CDX2 Information for Pathologists
Summary:
Homeobox gene that encodes a nuclear transcription factor critical for intestinal embryonic development; relatively specific for intestinal epithelium (but see positive staining below). Terminology Also called caudal-related homeobox gene 2, caudal type homeobox transcription factor 2. Homologue of Drosophila melanogaster homeobox gene-caudal. Uses by pathologists
Common Uses By Pathologists:
Fairly specific marker of GI origin for adenocarcinomas, but also stains selected adenocarcinomas of other sites indicated below (Am J Surg Pathol 2003;27:303); can use to determine origin of metastatic adenocarcinoma as part of panel (Arch Pathol Lab Med 2007;131:1561). Distinguish primary and secondary colorectal adenocarcinomas (Arch Pathol Lab Med 2005;129:920). Distinguish primary bladder adenocarcinoma (CDX2-, villin-) from colorectal carcinoma extending / metastatic to bladder (CDX2+, villin+, Mod Pathol 2005;18:1217). Distinguish mucinous bronchioloalveolar adenocarcinoma (CDX2-) from metastatic mucinous colorectal adenocarcinoma (CDX2+, Am J Clin Pathol 2004;122:421). Microscopic (histologic) images
| CDX2 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 33.5 kDa | |
| Chromosomal Location | |
| q12.2 [chr: 13] [chr_start: 27962137] [chr_end: 27971139] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDX2 |
| Entrez Gene ID | 1045 |
| RefSeq Protein Accession(s) | NP_001256 |
| RefSeq mRNA Accession(s) | XM_011534875; XM_011534878; NM_001265; XM_011534876; XM_011534879; NM_001354700 |
| RefSeq Genomic Accession(s) | NC_000013 |
| UniProt ID(s) | Q99626 |
| PharmGKB ID(s) | PA26352 |
| KEGG Gene ID(s) | hsa:1045 |
| General Description of CDX2 . | |
| Cdx2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The cdx2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that cdx2 is superior marker compared to CK20. | |
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