PDF DatasheetDiscontinued
Antibody (Suitable for clinical applications)
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Cdx2 Information for Pathologists
Summary:
Homeobox gene that encodes a nuclear transcription factor critical for intestinal embryonic development; relatively specific for intestinal epithelium (but see positive staining below). Terminology Also called caudal-related homeobox gene 2, caudal type homeobox transcription factor 2. Homologue of Drosophila melanogaster homeobox gene-caudal. Uses by pathologists
Common Uses By Pathologists:
Fairly specific marker of GI origin for adenocarcinomas, but also stains selected adenocarcinomas of other sites indicated below (Am J Surg Pathol 2003;27:303); can use to determine origin of metastatic adenocarcinoma as part of panel (Arch Pathol Lab Med 2007;131:1561). Distinguish primary and secondary colorectal adenocarcinomas (Arch Pathol Lab Med 2005;129:920). Distinguish primary bladder adenocarcinoma (CDX2-, villin-) from colorectal carcinoma extending / metastatic to bladder (CDX2+, villin+, Mod Pathol 2005;18:1217). Distinguish mucinous bronchioloalveolar adenocarcinoma (CDX2-) from metastatic mucinous colorectal adenocarcinoma (CDX2+, Am J Clin Pathol 2004;122:421). Microscopic (histologic) images
| Cdx2 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 33.5 kDa | |
| Chromosomal Location | |
| q12.2 [chr: 13] [chr_start: 27962137] [chr_end: 27971139] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CDX2 |
| Entrez Gene ID | 1045 |
| RefSeq Protein Accession(s) | NP_001256 |
| RefSeq mRNA Accession(s) | XM_011534875; XM_011534878; NM_001265; XM_011534876; XM_011534879; NM_001354700 |
| RefSeq Genomic Accession(s) | NC_000013 |
| UniProt ID(s) | Q99626 |
| PharmGKB ID(s) | PA26352 |
| KEGG Gene ID(s) | hsa:1045 |
| General Description of Cdx2 . | |
| The caudal-related homeodomain protein 2, CDX-2, is a transcription factor which is expressed in the intestine and is thought to play an important role in the proliferation and differentiation of intestinal epithelial cells. The CDX-2 protein is expressed in primary and metastatic colorectal carcinomas, intestinal metaplasia of the stomach and intestinal type gastric cancer. In human colorectal cancer, the expression of both CDX2 and carbonic anhydrase 1, a gene regulated by CDX2, is reduced or absent. CDX-2 is one of the important regulators in defining pathways for coordinate control of drug metabolism in the gastrointestinal tract. | |
There are no reviews yet.