PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Chromogranin A Information for Pathologists
Summary:
Granin protein located in secretory vesicles of neurons and endocrine cells (Clin Invest Med 1995;18:47, Endocr Rev 2011;32:755). Immunostain is specific but not sensitive for neuroendocrine cells; more sensitive in well differentiated vs. poorly differentiated tumors. Antibody binds acidic glycoproteins in the soluble fraction of neurosecretory granules. Serum levels may not be useful for diagnosis (Biomarkers 2012;17:186), but changes in levels may reflect response to therapy or recurrence (Folia Biol (Praha) 2011;57:173). Terminology
Common Uses By Pathologists:
Commonly used neuroendocrine marker (also synaptophysin and CD56) for normal cells and neuroendocrine tumors. Helps differentiate pheochromocytoma (almost always positive) from adrenocortical carcinoma (almost always negative, Am J Surg Pathol 2010;34:423). Microscopic (histologic) images Images hosted on PathOut server:.
| Chromogranin A General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 50.7 kDa | |
| Chromosomal Location | |
| [chr: CHR_HSCHR14_7_CTG1] [chr_start: 92923080] [chr_end: 92935293] [strand: 1]; q32.12 [chr: 14] [chr_start: 92923150] [chr_end: 92935285] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CHGA |
| Entrez Gene ID | 1113 |
| RefSeq Protein Accession(s) | XP_011534672; NP_001266; NP_001288619 |
| RefSeq mRNA Accession(s) | XM_011536370; NM_001301690; NM_001275 |
| RefSeq Genomic Accession(s) | NC_000014; NT_187601 |
| UniProt ID(s) | P10645 |
| PharmGKB ID(s) | PA26461 |
| KEGG Gene ID(s) | hsa:1113 |
| General Description of Chromogranin A . | |
| This antibody recognizes Chromogranin A. Chromogranin A (a protein of 439 amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. | |
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