Immunohistochemical staining of Chromogranin A of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

DISC: Chromogranin A [LK2H10] Antibody

SKU: QIVD99
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

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 PDF Datasheet

DISCONTINUED

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.

Application Notes

Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Chromogranin A Antibody Clone: LK2H10
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody LK2H10 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with human pheochromocytoma.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Chromogranin A Information for Pathologists

Summary:

Granin protein located in secretory vesicles of neurons and endocrine cells (Clin Invest Med 1995;18:47, Endocr Rev 2011;32:755). Immunostain is specific but not sensitive for neuroendocrine cells; more sensitive in well differentiated vs. poorly differentiated tumors. Antibody binds acidic glycoproteins in the soluble fraction of neurosecretory granules. Serum levels may not be useful for diagnosis (Biomarkers 2012;17:186), but changes in levels may reflect response to therapy or recurrence (Folia Biol (Praha) 2011;57:173). Terminology

Common Uses By Pathologists:

Commonly used neuroendocrine marker (also synaptophysin and CD56) for normal cells and neuroendocrine tumors. Helps differentiate pheochromocytoma (almost always positive) from adrenocortical carcinoma (almost always negative, Am J Surg Pathol 2010;34:423). Microscopic (histologic) images Images hosted on PathOut server:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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DISC: Chromogranin A [LK2H10] Antibody