PDF DatasheetHuman and Mouse Anti-Cytokeratin 10 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.1-0.2ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Cytokeratin 10 Antibody Clone: SPM261
Clone SPM261 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Cytokeratin 10 Positive Control Sample: A431, HeLa, MCF7 cells or Esophagus.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Skin extract of a human Psoriasis patient
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Cytokeratin 10 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin 10 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin and keratinocytes in skin. More moderate antibody staining intensity was present in epidermal cells in the skin and keratinocytes in skin. Low, but measureable presence of Cytokeratin 10 could be seen in. We were unable to detect Cytokeratin 10 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
| Cytokeratin 10 General Information | |
|---|---|
| Alternate Names | |
| Keratin, type I cytoskeletal 10, cytokeratin-10, CK-10, keratin-10, K10, KRT10 | |
| Molecular Weight | |
| 56.5kDa | |
| Chromosomal Location | |
| 17q21.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT10 |
| Entrez Gene ID | 3858 |
| Ensemble Gene ID | ENSG00000186395 |
| RefSeq Protein Accession(s) | XP_005257400, NP_000412 |
| RefSeq mRNA Accession(s) | NM_000421, XM_005257343 |
| RefSeq Genomic Accession(s) | NC_018928, NG_033147, NG_008405, NC_000017 |
| UniProt ID(s) | P13645 |
| UniGene ID(s) | P13645 |
| HGNC ID(s) | 6413 |
| Cosmic ID(s) | KRT10 |
| KEGG Gene ID(s) | hsa:3858 |
| PharmGKB ID(s) | PA30200 |
| General Description of Cytokeratin 10. | |
| This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers, increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger, more advanced tumors (greater than 2 cm, clinical stages II, III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. | |
Limitations and Warranty
enQuire Bio’s Cytokeratin 10 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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