Anti-Cytokeratin 18 Antibody Clone B23.1

$ 429.00

SKU: 3875-MSM9
Species: Human, Mouse (-), Rat (-), Hamster (-), Bovine (-), Canine (-), Pig (-), and Sheep (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: MS-QAVA™ Validated

 PDF Datasheet

Human, Mouse (-), Rat (-), Hamster (-), Bovine (-), Canine (-), Pig (-), and Sheep (-) Anti-Cytokeratin 18 Antibody Product Attributes

Species: Human, Mouse (-), Rat (-), Hamster (-), Bovine (-), Canine (-), Pig (-), and Sheep (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Cytokeratin 18 Antibody Clone: B23.1
Clone B23.1 Host and Isotype: Mouse IgG1
Anti-Human, Mouse (-), Rat (-), Hamster (-), Bovine (-), Canine (-), Pig (-), and Sheep (-) Cytokeratin 18 Positive Control Sample: MCF-7, HeLa, A431 cells or Breast Cancer
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: PMC-42 human breast carcinoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Cytokeratin 18 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Cytokeratin 18 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the tubules in kidney, exocrine glandular cells in the pancreas, glandular cells in the appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and trophoblastic cells in the placenta. Low, but measureable presence of Cytokeratin 18 could be seen in. We were unable to detect Cytokeratin 18 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin 18 expression as measured by anti-Cytokeratin 18 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ +++ ++ +++ +++ +++ +++ +++ +++ +++ ++ +++ +++ ++
KRT18 Variability ++ + +++ ++ + + ++ ++ ++ + + + + + ++ + + ++ + ++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
Size

Tag

Buffer and Stabilizer

,

Product Type

Host

Isotype

Applications

,

Species

Mass Spec Validated?

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Anti-Cytokeratin 18 Antibody Clone B23.1

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-3875-MSM9-P1-anti-Cytokeratin-18-antibody-300x271.png