PDF DatasheetAntibody (Suitable for clinical applications)
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Cytokeratin 7 Information for Pathologists
Summary:
Type II keratin of simple nonkeratinizing epithelia; molecular weight 51.4 kDa (OMIM: 148059 [Accessed 14 September 2018]). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. Essential features Membranous / cytoplasmic marker with expression in many normal epithelia and epithelial tumors. Despite wide distribution, useful as part of a panel in determining primary site of metastatic carcinoma.
Common Uses By Pathologists:
CK7 / CK20 profiles (general patterns, specificity varies):. CK7+ / CK20+: Extrahepatic carcinoma of the bile duct (majority) (Pathol Res Pract 2003;199:65). Gastric adenocarcinoma, subset (majority are CK7+ / CK20-) . Pancreatic adenocarcinoma, minor subset (co-expression associated with worse prognosis, however, majority are CK7+ / CK20-) (Cancer 2006;106:693).
| Cytokeratin 7 General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 51.4 kDa | |
| Chromosomal Location | |
| q13.13 [chr: 12] [chr_start: 52232520] [chr_end: 52252186] [strand: 1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT7 |
| Entrez Gene ID | 3855 |
| RefSeq Protein Accession(s) | NP_005547 |
| RefSeq mRNA Accession(s) | XR_001748699; XM_011538325; NM_005556; XR_001748700; XM_017019294 |
| RefSeq Genomic Accession(s) | NC_000012; NG_012296 |
| UniProt ID(s) | P08729 |
| PharmGKB ID(s) | PA30233 |
| KEGG Gene ID(s) | hsa:3855 |
| General Description of Cytokeratin 7 . | |
| Cytokeratin 7 (CK7) is a cytoplasmic intermediate filament protein expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointerstinal epithelium, prostate, hepatocyte and squamous epithelium. CK7 expression is absent in colon cancer, prostate cancer and squamous carcinomas. The restricted expression of CK7 in some epithelium makes it useful to identify the organ origin of adenocarcinomas when combined with staining of anti-Cytokeratin 20 and other cell specific markers. | |
![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 7 MS-QAVA™ monoclonal antibody [Clone: KRT7/903] (solid-line), reveals that less than 7.5% of signal is attributable to non-specific binding of anti-Cytokeratin 7 [Clone KRT7/903] to targets other than KRT7 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-3855-MSM3-P1-anti-Cytokeratin-7-antibody-150x150.png)
![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 7 MS-QAVA™ monoclonal antibody [Clone: KRT7/1499R] (solid-line), reveals that less than 13.6% of signal is attributable to non-specific binding of anti-Cytokeratin 7 [Clone KRT7/1499R] to targets other than KRT7 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-3855-RBM9-P0-anti-Cytokeratin-7-antibody-150x150.png)
![Analysis of Mass Spec data (dashed-line) of fractions stained with Cytokeratin 7 MS-QAVA™ monoclonal antibody [Clone: KRT7/760 + KRT7/903] (solid-line), reveals that less than 0.3% of signal is attributable to non-specific binding of anti-Cytokeratin 7 [Clone KRT7/760 + KRT7/903] to targets other than KRT7 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-3855-MSM5-P1-anti-Cytokeratin-7-antibody-150x150.png)
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