PDF DatasheetAntibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | HistoZyme |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Cytokeratin 8, Low Molecular Weight Antibody Clone: 35betaH11
Host and Isotype: Mouse IgM, kappa
Recommended Positive Control Sample: Skin, Colon
Cellular Localization of Antibody 35betaH11 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were immunized with cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Cytokeratin 8, Low Molecular Weight Information for Pathologists
| Cytokeratin 8, Low Molecular Weight General Information | |
|---|---|
| Alternate Names | |
| Molecular Weight | |
| 53.7 kDa | |
| Chromosomal Location | |
| q13.13 [chr: 12] [chr_start: 52897187] [chr_end: 52949954] [strand: -1] | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT8 |
| Entrez Gene ID | 3856 |
| RefSeq Protein Accession(s) | NP_002264; NP_001243222 |
| RefSeq mRNA Accession(s) | NR_045962; NM_001256282; NM_001256293; NM_002273 |
| RefSeq Genomic Accession(s) | NC_000012; NG_008402 |
| UniProt ID(s) | P05787 |
| PharmGKB ID(s) | PA30234 |
| KEGG Gene ID(s) | hsa:3856 |
| Associated Diseases (KEGG IDs) | Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension. {ECO:0000269|PubMed:12724528}. The disease is caused by mutations affecting the gene represented in this entry. |
| General Description of Cytokeratin 8, Low Molecular Weight . | |
| This antibody recognizes keratin polypeptide of 54 kDa. It stains strongly with non-squamous epithelium but does not stain squamous epithelium. It reacts with the sweat gland and ducts in the skin. Adenocarcinomas of the ovary, gastrointestinal tract, ductal carcinoma of breast, pancreas and bile ducts, stain positive with this antibody. | |
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
There are no reviews yet.