PDF DatasheetHuman, Monkey, Cow, Canine, Rabbit, Mouse, Rat, Chicken, and Turtle Anti-Cytokeratin, Acidic Antibody Product Attributes
Cytokeratin, Acidic Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-KRT77 antibody staining is expected to be primarily localized to the nuclear membrane and plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in Cytokeratin, Acidic antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in keratinocytes in skin and squamous epithelial cells in the oral mucosa. More moderate antibody staining intensity was present in keratinocytes in skin and squamous epithelial cells in the oral mucosa. Low, but measureable presence of Cytokeratin, Acidic could be seen inepidermal cells in the skin, Langerhans in skin, squamous epithelial cells in the cervix and uterine and esophagus. We were unable to detect Cytokeratin, Acidic in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Cytokeratin, Acidic expression as measured by anti-Cytokeratin, Acidic antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| KRT77 Variability | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | ++ | + | ++ | + | ++ |
| Cytokeratin, Acidic General Information | |
|---|---|
| Alternate Names | |
| KRT77, Keratin 77 | |
| Molecular Weight | |
| 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) | |
| Chromosomal Location | |
| 17q21.2 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | KRT77 |
| Entrez Gene ID | 374454 |
| Ensemble Gene ID | ENSG00000189182 |
| RefSeq Protein Accession(s) | NP_778253, XP_011536591, XP_011536590 |
| RefSeq mRNA Accession(s) | XM_011538288, NM_175078 |
| RefSeq Genomic Accession(s) | NC_000012, NC_018923, NG_012322 |
| UniProt ID(s) | Q7Z794, Q0IIN1 |
| UniGene ID(s) | Q7Z794, Q0IIN1 |
| HGNC ID(s) | 20411 |
| Cosmic ID(s) | KRT77 |
| KEGG Gene ID(s) | hsa:374454 |
| PharmGKB ID(s) | PA134896049 |
| General Description of Cytokeratin, Acidic. | |
| This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45,, 40kDa. MAb SPM116 recognizes the 65-67, 64, 59, 58, 56,, 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research, tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer,, esophageal cancer. | |
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